Prevalence of Multi-Drug Resistance Pseudomonas Aeruginosa in a Tertiary Care Hospital of Lahore

ABSTRACT

Pseudomonas is a negative bacterium and this bacterium is an important pathogen in health care centers. The goal of this observes is to determine the prevalence of multi-drug resistant (MDR) in Pseudomonas isolated from clinical specimens in a tertiary care clinic in Lahore. This research or have a look at turned into conducted within the microbiology branch within the tertiary care health center of Lahore from March 2021 to May 2021.CLED agar, nutrient agar, blood agar, and MacConkey agar were used in which samples were inoculated. Gram staining, catalase, oxidase, nitrate, urea, indole, lactose TSI reaction were the methods we use for the identification of Pseudomonas colonies. Kirby Bauers disc diffusion method was used in this study. In this method, MH (Muller-Hinton) agar was used after inoculation of colonies of Pseudomonas and after that swabbing occurs and specific antibiotics were punched on that agar and incubate all agar plates. After 24 hours of incubation zones around the antibiotics were identify the sensitivity and resistance of antibiotics. In this research, we took 53 samples of patients and in those 53 samples, 22 were found multi-drug resistant (MDR) in Pseudomonas. In this research, we use cefepime, ceftazidime, meropenem, amikacin, gentamicin, tobramycin, imipenem, ciprofloxacin antibiotics. The highest MDR become discovered against cefepime and the bottom MDR became observed against amikacin in order that amikacin became the effective antibiotic. Antibiotic-resistant Pseudomonas is rising as a crucial human health issue. Some preventions and measurements are required to overcome the issue. Efforts of researchers and healthcare professionals are required to train human beings about antibiotics use and other contamination control measures also.

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