Estimation and Comparison of Glucose, Urea, and Electrolytes in Sodium Fluoride, Plain and Glyceraldehyde Tube

ABSTRACT

Glucose is one of the most commonly analyzed specimen in the laboratory. There are different methods used to preserve glucose level in blood sample. Most commonly used method is collection of blood sample in a tube containing sodium fluoride. In current study another antiglycolytic agent, glyceraldehyde is compared with sodium fluoride tube and plain tube for its antiglycolytic effect and its interference in the measurement of other chemistry parameters. Objective: The specific objective of the study was to estimate and compare the Glucose, Urea and Electrolytes (sodium and Potassium) concentration from blood collected in three different blood collection vacutainers with different preservatives immediately and after 8-hours. Methods: GOD-PAP method was used for measurement of glucose. Urea was measured by using Urease UV method while sodium and potassium were measured by using ISE method. All the measurements were done on each sample in duplicate. Once immediately after collection and secondly after 8-hour storage at room temperature. Results: Initially all three tubes preserved blood glucose equally. While after 8 hours all the three tubes i.e., with and without anticoagulant did not preserve glucose levels. However, all other chemistry parameters (urea, sodium and potassium) remained preserve in both tubes. (GLY and PTC). Conclusion: It was concluded that all chemistry parameters can be measured from glyceraldehyde and plain tube while none of the tubes (including NaF, Glyceraldehyde and plain tube) was fit to store glucose samples for long period.

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